We all know that for every open fret, the fifth fret of the above string gives the same frequency (except for the 3rd string which gives the frequency of the 2nd string with the 4th fret). I want to know that if I tune my fat E string to any arbitrary frequency(not E) and tune the other strings in accordance with that, will my guitar be properly tuned? The 5th fret of every string certainly will be tuned with each other, but would I get correct notes for all frets and open frets as well? I hope I made myself clear. Please help.